![]() (1998) Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting for identification of Acinetobacter genomic species and typing of Acinetobacter baumannii. (1997) AFLP markers for DNA fingerprinting in cattle. (1998) Use of an Amplified Fragment Length Polymorphism technique to fingerprint and differentiate isolates of Heliobacter pylori. (2002) Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting. (2001) Amplified fragment length polymorphism (AFLP) protocol for genotyping the malarial parasite Plasmodium falciparum. (2000) A genetic linkage map of the apicomplexan parasite Eimeria tenella. (2002) Numerous, robust markers for Plasmodium chabaudi by the method of amplified fragment length polymorphism. Grech, K., Martinelli, A., Pathirana, S., et al. (2002) Genetic analysis of phenotype in Trypanosoma brucei: a classical approach to potentially complex traits. (2000) Amplified fragment length polymorphism in parasite genetics. (1996) Evaluation of the DNA fingerprinting method AFLP as a new tool for bacterial taxonomy. Janssen, P., Coopman, G., Huys, J., et al. (1996) A PCR-based DNA fingerprinting technique: AFLP for molecular typing of bacteria. (1995) AFLP: a new technique for DNA fingerprinting. This chapter describes the principles of AFLP and experimental procedures. Typically, the fragments detected by AFLP are inherited in Mendelian fashion as co-dominant markers, making the technique amenable to tracking inheritance of genetic loci in progeny from crossed lines of organisms, and in studies of population genetics. AFLP detects the presence of point mutations, insertions, deletions, and other genetic rearrangements. ![]() Therefore, it is very versatile and particularly valuable for organisms for which no substantive DNA sequence data are available. The technique can be applied to studies of DNA of any origin and complexity, without prior sequence knowledge. AFLP analysis combines the reliability of restriction enzyme digestion with the utility of the polymerase chain reaction. The amplified (restriction) fragment length polymorphism (AFLP) technique is a method for DNA profiling that is now widely applied for assessing diversity among various organisms with varying genomic complexity, from small bacterial to large plant genomes. ![]()
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